Novel action of lignans isolated from Hernandia nymphaeifolia on Ca²⁺ signaling in renal tubular cells

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• 作者：Chao ; Yu-Ying ; Chen ; Ih-Sheng ; Yeh ; Jwu-Lai ; Chen ; Jih-Jung ; Ko ; Ying-Chin ; Cheng ; Jin-Shiung ; et. al.
• 关键词：Lignan ; Ca²⁺ ; Ca²⁺ store ; Fura-2 ; MDCK cell ; Thapsigargin
• 刊名：European Journal of Pharmacology
• 出版年：2002
• 出版时间：May 17, 2002
• 年：2002
• 卷：443
• 期：1-3
• 页码：31-38
• 全文大小：323 K

文摘

The effect of five lignans, epi-aschantin, epi-magnolin, epi-yangambin, deoxypodophyllotoxin and yatein, isolated from Hernandia nymphaeifolia on Ca²⁺ signaling in Madin–Darby canine kidney cells was examined using fura-2 as a Ca²⁺ indicator. These lignans at concentrations between 10 and 100 μM increased [Ca²⁺]_i in a concentration-dependent manner. Removal of extracellular Ca²⁺ abolished the Ca²⁺ signals evoked by 50 μM of the lignans. La³⁺(50 μM) abolished the Ca²⁺ signals induced by 100 μM of epi-aschantin, epi-magnolin and epi-yangambin, and 20 μM deoxypodophyllotoxin, but inhibited by 60 % 50 μM yatein-induced responses. All five lignans (50–100 μM) inhibited by 42–65 % thapsigargin-induced capacitative Ca²⁺ entry, and inhibited by 23–61 % thapsigargin-induced intracellular Ca²⁺ release. Epi-yangambin (100 μM), epi-magnolin (100 μM), and epi-aschantin (100 μM) inhibited by 8–38 % 10 μM ATP-induced Ca²⁺ release. Trypan blue exclusion revealed that incubation with deoxypodophyllotoxin or yatein (but not the other lignans) decreased cell viability in a concentration-dependent manner. Together, the results suggest that, in renal tubular cells, these lignans exert multiple actions on Ca²⁺ signaling. They caused Ca²⁺ influx but reduced thapsigargin-induced capacitative Ca²⁺ entry and also thapsigargin- and ATP-induced Ca²⁺ release. Additionally, deoxypodophyllotoxin and yatein may be cytotoxic.