Protease-activated receptors expression in gingiva in periodontal health and disease

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• 作者：Diya Zhang^a ; Shenglai Li^b ; Lingjing Hu^c ; Lieping Sheng^a ; Zheng Cao^a ; Yanmin Wu^c ; Lili Chen^c ; ^{chenlili_1030@163.com" class="auth_mail}
• 关键词：Protease-activated receptor ; Gingiva ; Chronic periodontitis ; Immunohistochemistry ; Reverse transcriptase polymerase chain reaction
• 刊名：Archives of Oral Biology
• 出版年：April, 2014
• 年：2014
• 卷：59
• 期：4
• 页码：393-399
• 全文大小：1435 K

文摘

Objective

Protease-activated receptors (PARs) are a unique class of receptors which are implicated in mediating inflammation, pain and other functions. The aim of this study was to elucidate the role of PARs in the pathogenesis of chronic periodontitis by differential expression analysis of PARs in the gingival tissues of chronic periodontitis patients compared with those of healthy control individuals.

Design

Gingival tissue specimens were collected from chronic periodontitis patients (n = 20) and control individuals (n = 20). The expression of PAR-1, -2, -3 and -4 was determined in these tissues by immunohistochemistry and differential expression between the two groups was investigated by quantitative real-time reverse transcription-polymerase chain reaction analysis.

Results

PAR-1, -2, -3 and -4 were expressed in all gingival tissues. A significant overexpression of PAR-3 was detected in chronic periodontitis-affected tissues compared to healthy gingival tissues. However, expression of PAR-2 was decreased in periodontal lesions.

Conclusions

Our study shows that PAR-1, -2, -3 and -4 are expressed in both healthy and inflamed gingival tissues. Furthermore, PAR-2 and PAR-3 may contribute to the inflammatory responses associated with chronic periodontitis.