Selective encapsulation of hemoproteins from mammalian cells using mesoporous metal oxide nanoparticles

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• 作者：Mohamed Khairy ; Sherif A. El-Safty
• 关键词：Metal oxides ; Hemoproteins ; Selective encapsulation ; Mesoporous ; Mammalian cells
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2013
• 出版时间：1 November, 2013
• 年：2013
• 卷：111
• 期：Complete
• 页码：460-468
• 全文大小：2647 K

文摘

A key requirement in successful protein encapsulation is the fabrication of selective protein supercaptors that are not impeded by the physical shape and three-dimensional hydrodynamics of the protein, exhibit minimal clogging effect but with high protein retention, and with uniformly sized adsorbent pores. We report a novel nanomagnet-selective supercaptor approach in the encapsulation of hemoprotein from mammalian cells using mesoporous metal oxide nanoparticles (NPs). Different morphologies of mesoporous NiO and Fe₃O₄ NPs were fabricated. Among these nanoadsorbents, NiO nanoroses (NRs) had higher loading capacity of hemoprotein than NiO nanospheres (NSs) and nanoplatelets (NPLs), or even superparamagnetic Fe₃O₄ NPs. The key finding of this study was that mesoporous NiO nanomagnet supercaptors show exceptional encapsulation and selective separation of high-concentration Hb from human blood. In this induced-fit separation model, in addition to the heme group distributions and protein-carrier binding energy, the morphology and magnetic properties of NiO NPs had a key function in broadening the controlled immobilization affinity and selectivity of hemoproteins. In addition, thermodynamics, kinetics, and theoretical studies were carried out to investigate the optimal performance of protein adsorption.