Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels

详细信息    查看全文

• 作者：Pavel Novak ; Julia Gorelik ; Umesh Vivekan ; a ; Andrew?I. Shevchuk ; Yaroslav?S. Ermolyuk ; Russell?J. Bailey ; Andrew?J. Bushby ; Guy?W.J. Moss ; Dmitri?A. Rusakov ; David Klenerman ; Dimitri?M. Kullmann ; Kirill?E. Volynski ; Yuri?E. Korchev
• 刊名：Neuron
• 出版年：2013
• 出版时间：18 September, 2013
• 年：2013
• 卷：79
• 期：6
• 页码：1067-1077
• 全文大小：2165 K

文摘

| Figures/TablesFigures/Tables | ReferencesReferencesion=""1.0"" encoding=""UTF-8""?>

ass=""h3"">Summary

Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal?mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (¡Ü1?¦Ìm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100-150?nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K⁺, Na⁺, Cl^?, and Ca²⁺ channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.

ass=""h4"">Video Abstract

<a class=""cLink"" queryStr="""" href=""#mmc2"">