Site-specific integration and constitutive expression of key genes into Escherichia coli chromosome increases shikimic acid yields
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- 作者：Xianglei Liu ; Jun Lin ; junlin621@126.com" class="auth_mail" title="E-mail the corresponding author ; Haifeng Hu ; Bin Zhou ; Baoquan Zhu ; baoquanzhu@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Site-specific integration ; Constitutive expression ; Shikimic acid ; E. coli
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：96-104
- 全文大小：1281 K
文摘
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Site-specific integration of genes aroG, aroB, tktA and aroE at ptsHIcrr.
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Another key gene ppsA was integrated at tyrR.
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The genes could maintain stably and express constitutively without IPTG induction.
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Improvement of SA production and productivity using glucose–glycerol mixture.