Direct reprogramming of terminally differentiated B cells into erythroid lineage

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• 作者：Ken Sadahira ; Yumi Fukuchi ; Hiroyoshi Kunimono ; Masatoshi Sakurai ; Yasuo Ikeda ; Shinichiro Okamoto ; Hideaki Nakajima
• 关键词：B cell ; Reprogramming ; Erythroid ; Lineage conversion
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：19 October, 2012
• 年：2012
• 卷：586
• 期：20
• 页码：3645-3652
• 全文大小：1250 K

文摘

Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA-1, SCL together with CCAAT/enhancer binding protein (C/EBP) ¦Á turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid-specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.