Complete genome sequence and molecular phylogeny of a newfound hantavirus harbored by the Doucet鈥檚 musk shrew (Crocidura douceti) in Guinea

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• 作者：Se Hun Gu ; Violaine Nicolas ; Aude Lalis ; Nuankanya Sathirapongsasuti ; Richard Yanagihara
• 关键词：Hantavirus ; Crocidura ; Shrew ; Phylogeny ; Guinea
• 刊名：Infection, Genetics and Evolution
• 出版年：December, 2013
• 年：2013
• 卷：20
• 期：Complete
• 页码：118-123
• 全文大小：971 K

文摘

Elucidation of the molecular phylogeny of shrew-borne hantaviruses in sub-Saharan Africa has been hampered by the lack of full-length viral genomes. In this report, we present the complete genome analysis of a newfound hantavirus, designated Bow茅 virus, detected in ethanol-fixed intercostal muscle of a Doucet鈥檚 musk shrew (Crocidura douceti), captured in southwestern Guinea in February 2012. Full-length amino acid sequence comparison of the S-, M- and L-segment gene products revealed that Bow茅 virus differed by 24.1-53.4%, 17.0-59.9% and 14.6-39.7%, respectively, from all other representative rodent-, shrew- and mole-borne hantaviruses. Phylogenetic analysis, using maximum-likelihood and Bayesian methods, under the GTR + I + 螕 model of evolution, showed that Bow茅 virus shared a common ancestry with Tanganya virus, a hantavirus detected in the Therese鈥檚 shrew (Crocidura theresae) in Guinea. Whole genome analysis of many more hantaviruses from sub-Saharan Africa are needed to better clarify how the radiation of African shrews might have contributed to the phylogeography of hantaviruses.