Genotoxicity of ferric oxide nanoparticles in Raphanus sativus: Deciphering the role of signaling factors, oxidative stress and cell death

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• 作者：Quaiser Saquib¹ ; ² ; ^⁎⁎ ; ^{quaiser.saquib0@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Mohammad Faisal³ ; ^⁎⁎ ; ^{faisalm15@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Abdulrahman A. Alatar³ ; Abdulaziz A. Al-Khedhairy¹ ; Mukhtar Ahmed¹ ; Sabiha M. Ansari³ ; Hend A. Alwathnani³ ; Mohammad K. Okla³ ; Sourabh Dwivedi⁴ ; Javed Musarrat⁴ ; ⁵ ; Shelly Praveen⁶ ; Shams T. Khan¹ ; ² ; Rizwan Wahab¹ ; ² ; Maqsood A. Siddiqui¹ ; ² ; Javed Ahmad¹ ; ²
• 关键词：Ferric oxide nanoparticles ; Nanotoxicity ; DNA damage ; Apoptosis ; Oxidative stress ; Phytotoxicity
• 刊名：Journal of Environmental Sciences
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：47
• 期：Complete
• 页码：49-62
• 全文大小：2649 K

文摘

We have studied the genotoxic and apoptotic potential of ferric oxide nanoparticles (Fe₂O₃-NPs) in Raphanus sativus (radish). Fe₂O₃-NPs retarded the root length and seed germination in radish. Ultrathin sections of treated roots showed subcellular localization of Fe₂O₃-NPs, along with the appearance of damaged mitochondria and excessive vacuolization. Flow cytometric analysis of Fe₂O₃-NPs (1.0 mg/mL) treated groups exhibited 219.5%, 161%, 120.4% and 161.4% increase in intracellular reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), nitric oxide (NO) and Ca²⁺ influx in radish protoplasts. A concentration dependent increase in the antioxidative enzymes glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) has been recorded. Comet assay showed a concentration dependent increase in deoxyribonucleic acid (DNA) strand breaks in Fe₂O₃-NPs treated groups. Cell cycle analysis revealed 88.4% of cells in sub-G1 apoptotic phase, suggesting cell death in Fe₂O₃-NPs (2.0 mg/mL) treated group. Taking together, the genotoxicity induced by Fe₂O₃-NPs highlights the importance of environmental risk associated with improper disposal of nanoparticles (NPs) and radish can serve as a good indicator for measuring the phytotoxicity of NPs grown in NP-polluted environment.