- 作者：Silvia Maria Dí ; az Prado ; José ; Luis Ló ; pez Cedrú ; n ; Ramó ; n Luaces Rey ; Vanessa Medina Villaamil ; Augusto Á ; lvarez Garcí ; a ; Manuel Valladares Ayerbes ; Luis Antó ; n Apari
- 关键词：EGFR ; COX-2 ; p53 ; Oral leukoplakia ; Quantitative real-time PCR ; Molecular marker
- 刊名：Experimental and Molecular Pathology
- 出版年：2010
- 出版时间：October 2010
- 年：2010
- 卷：89
- 期：2
- 页码：197-203
- 全文大小：384 K
ObjectiveIdentify candidate SEBs (surrogate endpoint biomarkers) for premalignant trends in head and neck mucosa.Study design
Study, by qPCR (quantitative real-time polymerase chain reaction), the expression of COX-2, EGFR and p53 in 24 biopsies of non-dysplastic oral leukoplakia and contra-lateral normal-appearing mucosa.
Results
COX-2 was up-regulated in leukoplakia (79.2 % ); whereas EGFR and p53 were up-regulated (p > 0.05) in oral contra-lateral normal-appearing mucosa (60 % and 46 % respectively). Also, p53 expression was correlated with tobacco smoke habits and Spearman's rank correlation coefficient showed a positive linear correlation between p53 and EGFR mRNA expression levels.
Conclusions
COX-2 would serve as SEB of oral leukoplakia. The results suggest that p53 appears to be one of the molecular targets of tobacco-related carcinogens in leukoplakia and that the co-expression of p53 and EGFR may play a role in this kind of oral pre-cancerous lesion. More detailed studies of EGFR and p53 should be continued in the future.