Simultaneous determination of 15 aminoglycoside(s) residues in animal derived foods by automated solid-phase extraction and liquid chromatography-tandem mass spectrometry
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- 作者：Yanfei Tao ; Dongmei Chen ; Huan Yu ; Lingli Huang ; Zhaoying Liu ; Xiaoqin Cao ; Caixia Yan ; Yuanhu Pan ; Zhenli Liu ; Zonghui Yuan
- 关键词：Aminoglycosides ; Liquid chromatography&ndash ; tandem mass spectrometry ; Automated solid-phase extraction ; Weak cation-exchange
- 刊名：Food Chemistry
- 出版年：2012
- 出版时间：15 November, 2012
- 年：2012
- 卷：135
- 期：2
- 页码：676-683
- 全文大小：681 K
文摘
An automated method has been developed for the simultaneous quantification of 15 aminoglycosides in muscle, liver (pigs, chicken and cattle), kidney (pigs and cattle), cow milk, and hen eggs by liquid chromatography tandem mass spectrometry. Homogenized samples were extracted by monopotassium phosphate buffer (including ethylene diamine tetraacetic acid), and cleaned up with auto solid-phase extraction by carboxylic acid cartridges. The analytes were separated by a specialized column for aminoglycosides, and eluted with trifluoroacetic acid and acetonitrile. The decision limits (CC¦Á) of apramycin, gentamycin, tobramycin, paromomycin, hygromycin, neomycin, kanamycin, sisomicin, netilmicin, ribostamycin, kasugamycin, amikacin, streptomycin, dihydrostreptomycin and spectinomycin were ranged from 8.1 to 11.8 ¦Ìg/kg and detection capabilities (CC¦Â) from 16.4 to 21.8 ¦Ìg/kg. High correlation coefficients (r2 > 0.99) of calibration curves for the analytes were obtained within linear from 20 to 1000 ¦Ìg/kg. Reasonable recoveries (71-108 % ) were demonstrated with excellent relative standard deviation (RSD). This method is simple pretreatment, rapid determination and high sensitivity, which can be used in the determination of multi-aminoglycosides in complex samples.