Translation of a histone H3 tail as a model system for studying peptidyl-tRNA drop-off

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• 作者：Taek Jin Kang^a ; ^{taekkang@dongguk.edu"" rel=""nofollow} ; Hiroaki Suga^b ; ^c
• 关键词：Peptidyl-tRNA drop-off ; Translation ; Peptidyl-tRNA hydrolase
• 刊名：FEBS Letters
• 出版年：2011
• 出版时间：21 July 2011
• 年：2011
• 卷：585
• 期：14
• 页码：2269-2274
• 全文大小：892 K

文摘

We found that the synthesis of histone H3 N-terminal peptide (tail) in a reconstituted protein synthesis system yielded fragmented peptides along with the full-length product. With the combined use of MALDI-TOF analysis and peptidyl-tRNA hydrolase cleavage of the Flag tagged product species, we concluded that the fragments were generated by peptidyl-tRNA drop-off at specific sites and subsequent translation continuation. Using the histone H3 tail we also found that peptidyl-tRNA drop-off is strongly correlated with the amino acid context. We envision that the system described here would be useful as a model system for studying peptidyl-tRNA drop-off events.