Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders
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- 作者：Evgenia Platonovaa ; b ; Christian M. Winterflooda ; b ; Alexander Junemannc ; David Albrechta ; b ; Jan Faixc ; Helge Ewersa ; b ; d ; helge.ewers@fu-berlin.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Single-molecule localization microscopy (SMLM) ; PALM ; STORM ; Spectral-demixing direct stochastic reconstruction microscopy (SD-dSTORM) ; Single particle tracking (SPT) ; Nanobody ; Single domain antibody ; VHH
- 刊名：Methods
- 出版年：2015
- 出版时间：15 October 2015
- 年：2015
- 卷：88
- 期：Complete
- 页码：89-97
- 全文大小：1987 K
文摘
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Nanobodies are the epitope-binding domains of single chain antibodies.
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Nanobodies can be recombinantly expressed in bacteria and are thus easily available.
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A number of nanobodies against specific proteins and tags are available.
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Nanobodies provide better sample penetration than antibodies.
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Nanobodies deliver organic dyes closer to the target structure than antibodies.