Reconstitution of Targeted Deadenylation by the Ccr4-Not Complex and the YTH Domain Protein Mmi1
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- 作者：James A.W. Stowell1 ; Michael W. Webster1 ; Alexander Kö ; gel1 ; Jana Wolf1 ; Kathryn L. Shelley1 ; Lori A. Passmore1 ; 2 ; passmore@mrc-lmb.cam.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：gene expression ; poly(A) tail ; Ccr4-Not ; exonuclease ; RNA
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：17
- 期：8
- 页码：1978-1989
- 全文大小：2577 K
文摘
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We purify a fully recombinant S. pombe Ccr4-Not complex
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The complex has an intrinsic substrate preference, and both Ccr4 and Caf1 are active
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Ccr4-Not stably interacts with the YTH domain RNA-binding protein Mmi1
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Mmi1 accelerates deadenylation of RNAs by Ccr4-Not in a sequence-specific manner