Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery

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• 作者：Xu Qin^a ; Xuemei Xiang^a ; Xiaowen Sun^a ; ^b ; Hong Ni^b ; Lin Li^a ; ^{lilin@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：NPs ; nanoparticles ; SNPs ; silica nanoparticles ; SNP1 ; SiO2 NYSi40 nanoparticles ; SNP2 ; surface amino-grafted SNP1 ; SNP3 ; surface carboxylic-grafted SNP1 ; SNP4 ; surface aldehydic-grafted SNP1 ; SNPC ; composite nanoscale chitinase with Chi9602 and SNPs ; SNPC1 ; nanoscale chitinases of Chi9602 electrostatic adsorption on SNP1 ; SNPC2 ; nanoscale chitinases of Chi9602 electrostatic adsorption on SNP2 ; SNPC3 ; nanoscale chitinases of Chi9602 electrostatic adsorption on SNP3 ; SNPC4 ; nanoscale chitinases of
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：13-21
• 全文大小：2851 K

文摘

A series of amino, carboxylic, and aldehydic surface-grafted silica nanoparticles (SNPs) was prepared based on SiO₂ NYSi40 nanoparticles to develop an efficient, biocompatible, and cost-effective biopesticide delivery system. Bacillus thuringiensis chitinase (Chi9602) was immobilized onto SNP surface to prepare nanoscale chitinases (SNPCs) through electrostatic adsorption and covalent binding. The specimens were characterized by Fourier transform infrared, scanning electron microscopy, and zeta-potential analyses. The delivery capacity of the SNPs in Caenorhabditis elegans N2 was observed by immunofluorescence. Results demonstrated that amino-grafted SiO₂ nanoparticles with Chi9602 electrostatically adsorbed onto their surface (SNPC2) exhibited a relatively high enzyme immobilization rate (80.2%) and the highest (94.1%) residual enzyme activity among all SNPCs. SNPC2 also showed wider pH tolerance and relatively higher thermostability and ultraviolet radiation resistance capacity than Chi9602. Bioassays further showed that SNPC2 synergistically enhanced the nematicidal effect of B. thuringiensis YBT-020 preparation against C. elegans, with a reduced LC₅₀ of 8.35 mg/mL and a shortened LT₅₀ of 12.04 h. Immunofluorescence assays showed that SNPC2 had considerable delivery capacity to carry a large protein into C. elegans. Therefore, SNP2 can serve as an efficient nanocarrier for the delivery of macromolecular proteic biopesticides or drugs, indicating potential agricultural or biotechnological applications.