Dissection of antioxidant activity of durum wheat (Triticum durum Desf.) grains as evaluated by the new LOX/RNO method

详细信息    查看全文

• 作者：Maura Nicoletta Laus ; Damiana Tozzi ; Mario Soccio ; Aless ; ra Fratianni ; Gianfranco Panfili ; Donato Pastore
• 关键词：Antioxidant activity ; Durum wheat grains ; LOX/RNO method ; Synergism
• 刊名：Journal of Cereal Science
• 出版年：2012
• 出版时间：September, 2012
• 年：2012
• 卷：56
• 期：2
• 页码：214-222
• 全文大小：550 K

文摘

Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative LOX/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay, one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and lipophilic compounds were extracted from eight different whole flour samples; extracts were analyzed for AA and their content in several antioxidants. The LOX/RNO method measured very high AA values, with the highest ones [850-1500?¦Ìmol Trolox eq./g whole flour (dry weight)] for insoluble-bound phenolic extracts, highly correlated to total phenolic (r?=?0.761, P?<?0.001) and ferulic acid (r?=?0.816, P?<?0.001) contents. Hydrophilic and lipophilic extracts showed lower AA [70-140 and 40-60?¦Ìmol Trolox eq./g (dry weight), respectively], highly correlated to flavonoid (r?=?0.583, P?<?0.01) and protein (r?=?0.602, P?<?0.01), as well as ¦Â-tocotrienol (r?=?0.684, P?<?0.05) contents, respectively. Interestingly, the LOX/RNO method suggests that insoluble-bound phenolic compounds may exert very strong synergistic interactions within the extract. Contrarily, the TEAC assay did not correlate to any antioxidant content, resulted unable to highlight differences among samples, measured much lower AA values and did not suggest synergism. The use of the LOX/RNO method is useful to unearth new properties of phytochemicals from durum wheat grains, potentially giving health benefits.