Somatic cell recovery by microfiltration technologies: A novel strategy to study the actual impact of somatic cells on cheese matrix

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• 作者：Na Li^a ; ^b ; Romain Richoux^b ; Nadine Leconte^a ; Claudia Bevilacqua^c ; ^d ; Marie-Bernadette Maillard^a ; Sandrine Parayre^a ; Lydie Aubert-Frogerais^b ; Jessica Warlouzel^b ; Elisabeth Moya-Leclair^c ; Catherine Denis^c ; ^d ; Patrice Martin^c ; Valé ; rie Gagnaire^a ; ^{valerie.gagnaire@inra.fr}
• 刊名：International Dairy Journal
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：65
• 期：Complete
• 页码：5-13
• 全文大小：1418 K
• 卷排序：65

文摘

The actual impact of the somatic cells in the dairy technology is still ill-defined, because the increase in milk somatic cell count, usually correlated with mastitis factors, impairs the raw milk composition, through mainly unwanted proteolysis and lipolysis. This study used microfiltration technologies for recovering high quantity of somatic cells and for clarifying their role in cheese quality. Three series of Swiss-type cheeses were manufactured by adding 0 (control), 4 × 105 and 9 × 105 somatic cells mL−1. These cells were traced for the first time during the cheese making process by using adapted flow cytometry and real-time quantitative PCR. Proteolysis and lipolysis indices were measured throughout ripening time. Only a weak increase in lipolysis (+28%) and proteolysis (+8%) was observed in the highest somatic cell count cheese, despite 73% of the cells trapped within the cheeses. Our approach gives a new view of somatic cell role in cheese milk alteration.