The effect of oyster mushroom 尾-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.)
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- 作者：Radka Dob拧铆kov谩 ; Jana Blahov谩 ; Ivana Mikul铆kov谩 ; Helena Modr谩 ; Eva Pr谩拧kov谩 ; Zde艌ka Svobodov谩 ; Mi拧o 艩kori膷 ; Ji艡铆 Jarkovsk媒 ; Andrzej-Krzysztof Siwicki
- 关键词：Fish ; Condition factor ; Blood and plasma indices ; Chemiluminescence ; Immunoglobulins
- 刊名：Fish & Shellfish Immunology
- 出版年：December, 2013
- 年：2013
- 卷：35
- 期：6
- 页码：1813-1823
- 全文大小：1192 K
文摘
The aim of the study was to evaluate the effect of micronized 尾-1.3/1.6-D-glucan (BG) derived from the oyster mushroom Pleurotus ostreatus Hiratake and tetracycline antibiotic oxytetracycline (OTC) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (Cyprinus carpio L.). The fish tested were divided into five experimental groups and one control. Carp in the control group were fed commercial carp feed pellets. Fish in the five experimental groups were fed the same pellets supplemented with either OTC, a combination of OTC and BG, or BG as follows: 75聽mg聽oxytetracycline聽kg鈭? bw (OTC group), 75聽mg聽oxytetracycline聽kg鈭? bw and 0.5% 尾-glucan (OTC聽+聽0.5% BG group), 75聽mg聽oxytetracycline聽kg鈭? bw and 2.0% 尾-glucan (OTC聽+聽2.0% BG group), 0.5% 尾-glucan (0.5% BG group), and 2.0% 尾-glucan (2.0% BG group). OTC- and BG-supplemented diets and the control diet were administered to experimental and control carp for 50 days (i.e. samplings 1-3, the exposure period); for the following 14 days, fish were fed only control feed pellets with no OTC or BG supplementation (i.e. sampling 4, the recovery period). Blood and tissue samples were collected both during, and at the end of the study. No significant changes in biometrical indices (i.e. total length, standard length, total weight, hepatosomatic and spleen somatic index, and Fulton's condition factor) were found in experimental carp compared to control in any sampling. In haematological indices, significant changes were found only in sampling 2, in which shifts in PCV (P聽<聽0.01), Hb (P聽<聽0.01), and WBC (P聽<聽0.01), and in the counts of lymphocytes (P聽<聽0.01), monocytes (P聽<聽0.01), and neutrophil granulocytes-segments (P聽<聽0.05) were revealed. As for biochemical profiling, plasma concentrations of glucose, albumins, cholesterol, natrium, and chlorides (all P聽<聽0.01), and total proteins, lactate, phosphorus, and potassium (all P聽<聽0.05) as well as the catalytic activity of ALP (P聽<聽0.05) were altered in common carp. A significant change in induced (opsonizedzymosan particles, OZP) chemiluminescence (P聽<聽0.05) in sampling 3 and no shifts in serum immunoglobulins concentration were found in the immunological analysis. Histopathological examination of skin, gills, liver, spleen, and cranial and caudal kidneys revealed no obvious specific changes in any tissue analysed. The use of 尾-glucans in clinically healthy aquaculture remains an issue. Nevertheless, their use in breeding endangered by stress stimuli, infectious disease, or adverse environmental factors is defensible.