Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis

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• 作者：Pottathil Shinu ; Varsha Singh ; Anroop Nair ; Priya Mehrishi ; Sonia Mehta ; Ekta Joshi
• 关键词：Pulmonary tuberculosis ; Direct microscopy ; Cetylpyridinium chloride ; N-acetyl L-Cystine ; Decontamination
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：77
• 期：2
• 页码：150-157
• 全文大小：312 K

文摘

The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected cases of pulmonary tuberculosis. To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 796) were studied (culturing on L?wenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, specificity, negative predictive value, and positive predictive value of 97.99 % , 87.53 % , 70.19 % , and 99.32 % , respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected significantly higher number of MTB cases (n = 208) than NALC-NaOH decontamination method (n = 149) particularly in sputum with scanty bacilli and smear-negative cases, indicating the potential of CPC-NaCl decontamination method to preserve paucibacillary cases more efficient than NALC-NaOH decontamination method.