Acute and long-term effects of botulinum neurotoxin on the function and structure of developing extraocular muscles

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• 作者：Scott A. Croes ; Larisa M. Baryshnikova ; Soniya S. Kaluskar ; Christopher S. von Bartheld
• 关键词：Oculomotor ; Motor endplate ; Neuromuscular junction ; Eye muscle ; Contractile force ; Mitochondria ; Botulinum toxin ; Strabismus
• 刊名：Neurobiology of Disease
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：25
• 期：3
• 页码：649-664
• 全文大小：4899 K

文摘

Strabismus is a misalignment of the visual axes, due to an imbalance in extraocular muscle (EOM) function. Botulinum neurotoxin (BoNT) treatment can correct the misalignment with permanent therapeutic effects in infants, possibly because the toxin causes structural alterations in developing EOM. To determine whether BoNT indeed permanently weakens developing EOMs, we examined the chicken oculomotor system. Following injections of BoNT in hatchling chicks, we quantified physiological parameters (contractile force measurements) and morphological parameters (myofiber morphometry, innervation, quantitative transmission electron microscopy of mitochondria/fiber types). Treatment of developing EOM with BoNT caused acute reductions of muscle strength and mitochondrial densities, but minimal changes in muscle fiber diameter and neuromuscular junction structures. Contrary to expectations, contractile force was fully recovered by 3–4 months after treatment. Thus, permanent therapeutic effects of BoNT most likely do not cause permanent changes at the level of the peripheral effector organ, but rather involve central (CNS) adaptive responses.