Copy number variation of the APC gene is associated with regulation of bone mineral density

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• 作者：Shelby Chew ; Zari Dastani ; Suzanne J. Brown ; Joshua R. Lewis ; Frank Dudbridge ; Nicole Soranzo ; Gabriela L. Surdulescu ; J. Brent Richards ; Tim D. Spector ; Scott G. Wilson
• 关键词：Bone mineral density ; Osteoporosis ; Copy number variation ; APC ; Association
• 刊名：Bone
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：51
• 期：5
• 页码：939-943
• 全文大小：368 K

文摘

Introduction

Genetic studies of osteoporosis have commonly examined SNPs in candidate genes or whole genome analyses, but insertions and deletions of DNA, collectively called copy number variations (CNVs), also comprise a large amount of the genetic variability between individuals. Previously, SNPs in the APC gene have been strongly associated with femoral neck and lumbar spine volumetric bone mineral density in older men. In addition, familial adenomatous polyposis patients carrying heterozygous mutations in the APC gene have been shown to have significantly higher mean bone mineral density than age- and sex-matched controls suggesting the importance of this gene in regulating bone mineral density. We examined CNV within the APC gene region to test for association with bone mineral density.

Methods

DNA was extracted from venous blood, genotyped using the Human Hap610 arrays and CNV determined from the fluorescence intensity data in 2070 Caucasian men and women aged 47.0 ¡À 13.0 (mean ¡À SD) years, to assess the effects of the CNV on bone mineral density at the forearm, spine and total hip sites.

Results

Data for covariate adjusted bone mineral density from subjects grouped by APC CNV genotype showed significant difference (P = 0.02-0.002). Subjects with a single copy loss of APC had a 7.95 % , 13.10 % and 13.36 % increase in bone mineral density at the forearm, spine and total hip sites respectively, compared to subjects with two copies of the APC gene.

Conclusions

These data support previous findings of APC regulating bone mineral density and demonstrate that a novel CNV of the APC gene is significantly associated with bone mineral density in Caucasian men and women.