Assessing mechanisms of toxicant response in the amphipod Melita plumulosa through transcriptomic profiling
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- 作者：Sharon E. Hook ; Hannah L. Osborn ; David A. Spadaro ; Stuart L. Simpson
- 关键词：Amphipod ; Gene expression ; Genomics ; Metals ; Microarray ; Oil ; Insecticides ; Mode of action
- 刊名：Aquatic Toxicology
- 出版年：January, 2014
- 年：2014
- 卷：146
- 期：Complete
- 页码：247-257
- 全文大小：2671 K
文摘
This study describes the function of transcripts with altered abundance in the epibenthic amphipod, Melita plumulosa, following whole-sediment exposure to a series of common environmental contaminants. M. plumulosa were exposed for 48 h to sediments spiked and equilibrated with the following contaminants at concentrations predicted to cause sublethal effects to reproduction: porewater ammonia 30 mg L鈭?; bifenthrin at 100 渭g kg鈭?; fipronil at 50 渭g kg鈭?; 0.6% diesel; 0.3% crude oil; 250 mg Cu kg鈭?; 400 mg Ni kg鈭?; and 400 mg Zn kg鈭?. RNA was extracted and hybridized against a custom Agilent microarray developed for this species. Although the microarray represented a partial transcriptome and not all features on the array could be annotated, unique transcriptomic profiles were generated for each of the contaminant exposures. Hierarchical clustering grouped the expression profiles together by contaminant class, with copper and zinc, the petroleum products and nickel, and the pesticides each forming a distinct cluster. Many of the transcriptional changes observed were consistent with patterns previously described in other crustaceans. The changes in the transcriptome demonstrated that contaminant exposure caused changes in digestive function, growth and moulting, and the cytoskeleton following metal exposure, whereas exposure to petroleum products caused changes in carbohydrate metabolism, xenobiotic metabolism and hormone cycling. Functional analysis of these gene expression profiles can provide a better understanding of modes of toxic action and permits the prediction of mixture effects within contaminated ecosystems.