Identification of I. ricinus, I. persulcatus and I. trianguliceps species by multiplex PCR

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• 作者：Kairi Vä ; rv^a ; ^b ; ^c ; ^{kairi.varv@tai.ee} ; Anna Ivanova^a ; Julia Geller^a ; Jaanus Remm^d ; Kertu Jaik^d ; Nina Tikunova^e ; Vera Rar^e ; Å ; ke Lundkvist^c ; Irina Golovljova^a ; ^c ; ^e ; ^f
• 关键词：I. ricinus ; I. persulcatus ; I. trianguliceps ; ITS2 ; Species identification
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：8
• 期：2
• 页码：235-240
• 全文大小：1064 K
• 卷排序：8

文摘

Correct identification of tick species is an essential requirement for any scientific study engaged in tick-associated research. However, morphological identification can lead to misinterpretations, especially when dealing with vector-host research and sub-adult, engorged or damaged specimens. To overcome this limitation, we developed a novel assay to discriminate between Ixodes ricinus, I. persulcatus and I. trianguliceps species collected from rodents or vegetation, using the second internal transcribed spacer (ITS2) as a genetic marker. This single tube multiplex PCR allows specific amplification of targeted species and produces rapid and accurate results. The specificity was confirmed by sequencing the ITS2 and partial 16S rRNA genes from ticks collected from Estonia, Latvia, Sweden and Russia. We tested the assay in a large-scale experiment, and a total of 1284 ticks removed from rodents and shrews were successfully identified at species level.