Liquid chromatography-tandem mass spectrometric analysis of ten estrogen metabolites at sub-picogram levels in breast cancer women
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- 作者：Alaa Khedra ; akhedr@kau.edu.sa" class="auth_mail" title="E-mail the corresponding author ; khedr1961@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Abdulrahman M. Alahdalb
- 关键词：EMs ; estrogen metabolites ; BMP ; 3-hydroxymethyl-propyphenazone ; Dns-Cl ; dansyl chloride ; IT-MS ; ion trap mass spectrometer ; QQQ-MS ; triple quadrupole mass spectrometer
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：1031
- 期：Complete
- 页码：181-188
- 全文大小：1055 K
文摘
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Improved LC–ESI-MS method for determination of estrogens and estrogen metabolites in plasma.
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Detection of sub-picogram levels of catechol estrogens in women plasma.
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ESI-ionization suppression due to reagent was minimized by reacting the excess BMP with methanol.
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The method could be applicable for diagnosis of women candidate to breast cancer.
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The proposed procedure is more sensitive and showed less ESI-ionization suppression effect, than commonly applied dansylation method.