Monitoring utilizations of amino acids and vitamins in culture media and Chinese hamster ovary cells by liquid chromatography tandem mass spectrometry
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- 作者：Jinshu Qiu ; jqiu@amgen.com" class="auth_mail" title="E-mail the corresponding author ; qiujinshu@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Pik Kay Chan ; Pavel V. Bondarenko
- 关键词：CE ; collision energy ; ESI ; electrospray ionization ; HFBA ; heptaflurobutyric acid ; IS ; internal standard ; LC ; liquid chromatography ; LC&ndash ; MS/MS ; liquid chromatography tandem mass spectrometry ; LOQ ; limit of quantitation ; MS ; mass spectrometry ; QQQ ; triple-quadrupole mass spectrometer ; RT ; retention time ; RSD ; relative standard deviation ; SRM ; selected reaction monitoring
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：5 January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：163-172
- 全文大小：1069 K
文摘
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A LC/MS/MS method was developed to quantify 30 metabolites in media and CHO cells.
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Essential and non-essential amino acids were separated by fed-batch feeding.
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Ten essential amino acids showed a zig–zag pattern in response to feeding.
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Seven non-essential amino acids showed smoother profiles with wide changes.
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Five vitamins accumulated but 4 changed little during a culture period.