Liquid chromatography-tandem mass spectrometric determination of propofol in rat serum and hair at attogram level after derivatization with 3-bromomethyl-propyphenazone

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• 作者：Alaa Khedr^a ; ^{akhedr@kau.edu.sa} ; ^{khedr1961@gmail.com} ; Soad S.Abd El-Hay^a ; ^b ; Ahmed K. Kammoun^a
• 关键词：BMP ; 3-bromomethyl-propyphenazone ; PRO ; propofol ; EUG ; eugenol ; IT-MS ; ion trap mass spectrometer ; QQQ-MS ; triple quadrupole mass spectrometer
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：195-202
• 全文大小：1250 K
• 卷排序：134

文摘

Derivatization of propofol with 3-bromomethyl-propyphenazone followed by LVC/MS analysis. The derivatized propofol was detectable at attogram level in hair and serum. The LLOQ was 0.01 pg/mL serum (equivalent to 50 ag/μL in injectable solution). The ionization suppression was minimized by deactivating the excess reagent with methanol. The concentration patterns of propofol in rat serum and hair were unmatched.