The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells

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• 作者：Julie Nigro ; Jacinta F. White ; John A.M. Ramshaw ; David N. Haylock ; Susan K. Nilsson ; Jerome A. Werkmeister
• 关键词：Mesenchymal stem cell ; Bioreactor ; Glycosaminoglycan ; Cell proliferation ; Bone marrow ; Cell adhesion
• 刊名：Biomaterials
• 出版年：2010
• 出版时间：July 2010
• 年：2010
• 卷：31
• 期：21
• 页码：5689-5699
• 全文大小：9451 K

文摘

There is a large biomanufacturing and clinical need for cost-effective and simple techniques to expand mesenchymal stem cells whilst retaining their multipotency. Endosteum-derived particles were prepared, characterised and examined as a biomaterial to facilitate the in vitro expansion of human mesenchymal stem cells. Bovine endosteum-derived particles are composed of chondroitin sulphate glycosaminoglycans with 4- and 6-sulphation and N-sulphated heparan sulphate glycosaminoglycans. The particles were positive for perlecan, laminin and fibronectin by immunohistochemistry and α-mannose, α-glucose, terminal N-acetyl-α-d-glucosamine, N-acetyl-α-galactosamine and α-fucose, using lectin binding. Human mesenchymal stem cells showed greater than 96 % attachment to the particles after one day in spinner culture. After 7 days, the stem cells on decalcified particles were viable and had a 5-fold higher growth than the stem cells grown on Cytodex-2 beads. Significantly more stem cells were recovered from decalcified particles compared with mineralised particles (P < 0.05). Differentiation to chondrogenic, osteogenic and adipogenic lineages was maintained after culturing stem cells on the demineralised particles. We conclude that bovine endosteum-derived particles can be extracted from bone marrow to retain sulphated proteoglycans and glycosylated proteins. These particles are a suitable biomaterial for supporting the growth and retaining the multipotency of human mesenchymal stem cells.