Structural Basis for the Interaction between the IUS-SPRY Domain of RanBPM and DDX-4 in Germ Cell Development
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- 作者：Seung Kon Hong1 ; Kook-Han Kim1 ; 3 ; Eun Joo Song2 ; Eunice EunKyeong Kim1 ; eunice@kist.re.kr" class="auth_mail" title="E-mail the corresponding author ; eunice.kist@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：RanBP ; Ran binding protein ; RanBPM ; Ran binding protein M ; SPRY ; SPIa and the ryanodine receptor ; IUS ; immediate upstream to SPRY ; DDX-4 ; DEAD-box containing ATP-dependent RNA helicase ; ITC ; isothermal titration calorimetry ; SPSB1 ; SPRY domain-containing SOCS box protein GUSTAVUS ; PDB ; Protein Data Bank ; NES ; nuclear export signal ; SeMet ; selenomethionine ; PEG ; polyethylene glycol
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：23 October 2016
- 年：2016
- 卷：428
- 期：21
- 页码：4330-4344
- 全文大小：2052 K
文摘
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Immediate upstream to SPRY-SPRY domain forms a contiguous β-sandwich fold with two helices on each side.
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Highly conserved, unique shallow binding surface is formed on the surface of β-sheet.
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Peptide of DDX-4 RNA helicase binds to this surface of RanBPM.
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N-terminal α1 helix resembles a nuclear export signal for the nuclear export of RanBPM and a partner.