Early-Onset Hypertrophic Cardiomyopathy Mutations Significantly Increase the Velocity, Force, and Actin-Activated ATPase Activity of Human β-Cardiac Myosin

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• 作者：Arjun S. Adhikari¹ ; ² ; Kristina B. Kooiker² ; ³ ; Saswata S. Sarkar¹ ; Chao Liu¹ ; Daniel Bernstein² ; ³ ; James A. Spudich¹ ; ² ; Kathleen M. Ruppel¹ ; ² ; ³ ; ⁴ ; ^{kruppel@stanford.edu}
• 关键词：myosin ; hypertrophic cardiomyopathy ; HCM ; molecular motors ; in vitro motility ; single molecule
• 刊名：Cell Reports
• 出版年：2016
• 出版时间：13 December 2016
• 年：2016
• 卷：17
• 期：11
• 页码：2857-2864
• 全文大小：2108 K
• 卷排序：17

文摘

Early-onset HCM mutations increase myosin biomechanical functions D239N shows a large increase in velocity as compared to wild-type myosin H251N lowers S1-S2 binding affinity, which may increase available active myosin heads