Cloning, prokaryotic expression and functional analysis of squalene synthase (SQS) in Magnolia officinalis
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- 作者：Liangping Zhaa ; b ; 1 ; Shuang Liua ; c ; 1 ; Ping Sua ; Yuan Yuana ; y_yuan0732@163.com" class="auth_mail" title="E-mail the corresponding author ; Luqi Huanga ; huangluqi01@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Magnolia officinalis ; Squalene synthase ; Terpenoids ; Prokaryotic expression
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：28-34
- 全文大小：2155 K
文摘
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We cloned a full-length cDNA of squalene synthase gene from Magnolia officinalis for the first time.
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Prokaryotic expression showed that MoSQS mutant (MoSQSΔTM) could be expressed solubly in Escherichia coli Transetta (DE3).
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MoSQSΔTM is functional and provided an important base for the study of triterpenoids biosynthesis in Magnolia officinalis.