Mapping a kingdom-specific functional domain of squalene synthase
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- 作者：Kristin B. Linscotta ; 1 ; Thomas D. Niehausb ; 1 ; 2 ; Xun Zhuangb ; 1 ; Stephen A. Bellb ; 3 ; Joe Chappella ; b ; chappell@uky.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：SQS ; squalene synthase ; FPP ; farnesyl diphosphate ; PSPP ; presqualene diphosphate ; PSOH ; presqualene alcohol ; MSTFA ; N-Methyl-N-(trimethylsilyl) trifluoroacetamide ; GC-MS ; gas chromatography&ndash ; mass spectrometry
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1861
- 期：9
- 页码：1049-1057
- 全文大小：1607 K
文摘
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Non-fungal squalene synthase genes cannot complement the knockout mutation in yeast because a toxic intermediate accumulates
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A fungal hinge domain is necessary and sufficient foreach squalene synthase gene to complement the knockout mutation
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The 26-amino acid hinge motif is highly conserved and unique to each kingdom of life
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Over-expression of any fungal hinge domain, but not that from plants or animals, inhibits the growth of wild-type yeast