Mitochondria are the functional intracellular target for a photosensitizing boronated porphyrin

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• 作者：Munday ; Adam D. ; Sriratana ; Absorn ; Hill ; John S. ; Kahl ; Steven B. ; Nagley ; Phillip
• 关键词：Mitochondrion ; Photosensitiser ; Porphyrin ; Cell killing ; Oxidative stress ; Human cultured cell
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：1996
• 出版时间：March 27, 1996
• 年：1996
• 卷：1311
• 期：1
• 页码：1-4
• 全文大小：415 K

文摘

A photosensitizing boron-containing porphyrin derivative denoted BOPP, which is selectively localised into mitochondria, has been tested on Namalwa cells, in each of two genetic configurations: ρ⁺ cells containing normal mtDNA and mitochondrial respiratory functions, or ρ⁰ cells lacking mtDNA and devoid of mitochondrial oxidative phosphorylation. After short-term cellular uptake for 18 h, BOPP (30 μg/ml) was not cytotoxic, but did show marked phototoxicity in Namalwa ρ⁺ cells, concomitant with substantial reduction of mitochondrial respiratory activity. After long-term (3 days or more) exposure to BOPP without light, growth of Namalwa ρ⁺ cells was inhibited at concentrations significantly above 30 μg/ml. At such concentrations BOPP was shown to have direct inhibitory effects on mitochondrial azide-sensitive respiration of ρ⁺ cells. By contrast, BOPP showed neither cytotoxic nor phototoxic effects in ρ⁰ cells. These results indicate functional mitochondria to be a major cellular target in vivo after BOPP uptake and photoactivation.