- 作者：Stefan Rieken ; M.D.&lowast ; ; Stefan.Rieken@med.uni-heidelberg.de ; Daniel Habermehl ; M.D.&lowast ; ; Lena Wuerth ; B.T.A.&lowast ; ; Stephan Brons ; Ph.D.&dagger ; ; Angela Mohr ; M.D.&lowast ; ; Katja Lindel ; M.D.&lowast ; ; Klaus Weber ; Ph.D.&lowast ; ; Thomas Haberer ; Ph.D.&dagger ; ; Jü ; rgen Debus ; M.D. ; Ph.D.&lowast ; ; Stephanie E. Combs ; M.D.&lowast ;
- 关键词：Glioma ; Integrin ; Migration ; Particle therapy ; Radiotherapy
- 刊名：International Journal of Radiation Oncology*Biology*Physics
- 出版年：2012
- 出版时间：1 May, 2012
- 年：2012
- 卷：83
- 期：1
- 页码：394-399
- 全文大小：703 K
Purpose
To investigate the effect of carbon ion irradiation on glioma cell migration.Methods and Materials
U87 and Ln229 glioma cells were irradiated with photons and carbon ions. Migration was analyzed 24 h after irradiation. Fluorescence-activated cell sorting analysis was performed in order to quantify surface expression of integrins.
Results
Single photon doses of 2 Gy and 10 Gy enhanced ¦Á¦Í¦Â3 and ¦Á¦Í¦Â5 integrin expression and caused tumor cell hypermigration on both vitronectin (Vn) and fibronectin (Fn). Compared to integrin expression in unirradiated cells, carbon ion irradiation caused decreased integrin expression and inhibited cell migration on both Vn and Fn.
Conclusion
Photon radiotherapy (RT) enhances the risk of tumor cell migration and subsequently promotes locoregional spread via photon induction of integrin expression. In contrast to photon RT, carbon ion RT causes decreased integrin expression and suppresses glioma cell migration on both Vn and Fn, thus promising improved local control.