Cholesterol perturbing agents inhibit NMDA-dependent calcium influx in rat hippocampal primary culture
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- 作者：Frank ; Claudio ; Giammarioli ; Anna Maria ; Pepponi ; Rita ; Fiorentini ; Carla ; Rufini ; Stefano
- 关键词：AUC ; area under curve ; Cdex ; methyl-β ; -cyclodextrin ; NMDA-R ; NMDA-receptors ; NMDA-receptor ; Filipin ; Methyl-β ; -cyclodextrin ; Hippocampal cell ; Lipid raft
- 刊名：FEBS Letters
- 出版年：2004
- 出版时间：May 21, 2004
- 年：2004
- 卷：566
- 期：1-3
- 页码：25-29
- 全文大小：608 K
文摘
The present study was carried out to investigate the potential involvement of cholesterol-rich membrane microdomains in the mobilization of calcium induced by NMDA-receptors (NMDA-R). We herein provide evidence that agents interfering with plasma membrane cholesterol (namely, filipin and methyl-β-cyclodextrin (Cdex)) inhibit the NMDA-stimulated influx of calcium in hippocampal cells in culture. Filipin-treated cells maintained their morphology and were able to respond with a calcium influx to high K+ challenge, whereas Cdex altered both cellular parameters. These results suggest that the NMDA-R can be located in cholesterol-rich membrane microdomains or alternatively that the mechanisms coupling their dynamics in the post-synaptic membrane are dependent on the integrity of the microdomains.