Glutamate-induced calcium increase in myotubes depends on up-regulation of a sodium-dependent transporter

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• 作者：Frank ; Claudio ; Giammarioli ; Anna Maria ; Falzano ; Loredana ; Fiorentini ; Carla ; Rufini ; Stefano
• 关键词：Myogenic cell ; Adenosine ; Glutamate transporter ; Calcium ; 2CA ; 2-chloro-adenosine ; Glu ; glutamate ; PDC ; L-trans-pyrrolidine-2 ; 4-dicarboxylic acid ; THA ; DL-threo-b-hydroxyaspartic acid ; EAATs ; excitatory amino acid transporters
• 刊名：FEBS Letters
• 出版年：2002
• 出版时间：September 11, 2002
• 年：2002
• 卷：527
• 期：1-3
• 页码：269-273
• 全文大小：189 K

文摘

We report a study on the regulation by 2-chloro adenosine (2CA) of a glutamate (Glu) transporter in myogenic C2C12 cells. Long-term 2CA exposition significantly increased the V_max of the Glu transporter. Moreover, 2CA-treated cells responded to Glu challenge by a rapid and transient increase in their intracellular calcium level. The above reported effects were totally abolished by treating C2C12 cells with the Na⁺-dependent Glu transporter inhibitors DL-threo-b-hydroxyaspartic acid and L-trans-pyrrolidine-2,4-dicarboxylic acid. We propose that the possible link between the Glu uptake increase and the Glu induction of calcium rise could be the depolarizing currents carried by Na⁺ coupled with transporter activity.