Characterization of the interaction of human 5-lipoxygenase with its activating protein FLAP
详细信息 查看全文
- 作者：Ann-Kathrin Hä ; fnera ; Jana Gerstmeierb ; Michael Hö ; rniga ; Sven Georgea ; Ann-Katrin Balla ; Mirjam Schrö ; dera ; Ulrike Garschab ; Oliver Werzb ; Dieter Steinhilbera ; steinhilber@em.uni-frankfurt.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：5-H(p)ETE ; 5S-hydro(pero)xy-6 ; 8 ; 11 ; 14(E ; Z ; Z ; Z)-eicosatetraenoic acid ; 6-trans-LTB4 ; 5S ; 12R-dihydroxy-6 ; 8 ; 10 ; 14(E ; E ; E ; Z)-eicosatetraenoic acid ; 6-trans-12-epi-LTB4 ; 5S ; 12S-dihydroxy-6 ; 8 ; 10 ; 14-(E ; E ; E ; Z)-eicosatetraenoic acid ; Aa ; amino acid ; AA ; arachidonic acid ; DAPI ; diamidino-2-phenylindole ; FLAP ; 5-lipoxygenase-activating protein ; GSH ; reduced glutathione ; LO ; lipoxygenase ; LT ; leukotriene ; LTB4 ; leukotriene B4 (5S ; 12R-dihydroxy-6 ; 8 ; 10 ; 14(Z ; E ; E ; Z)-eicosatetraenoic acid) ; PBS ; Dulbecco's phosph
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：1851
- 期：11
- 页码：1465-1472
- 全文大小：789 K
文摘
- •
Glutathionylation of recombinant 5-LO does not influence product ratio.
- •
Diamide prevents cellular 5-LO and FLAP colocalization.
- •
Mutation of Cys 159, 300, 416 and 418 to Ser restores colocalization in diamide-treated cells.