Effects of Bni5 Binding on Septin Filament Organization

详细信息    查看全文

• 作者：Elizabeth A. Booth¹ ; ⁵ ; ^&dagger ; ; ^{elizabeth.booth@Grifols.com} ; Sarah M. Sterling¹ ; ^&dagger ; ; ^{smsterling@berkeley.edu} ; Dustin Dovala² ; ⁶ ; ^{dustin.dovala@novartis.com} ; Eva Nogales¹ ; ³ ; ⁴ ; ^{ENogales@lbl.gov} ; Jeremy Thorner¹ ; ^{jthorner@berkeley.edu}
• 关键词：AF555 ; Alexa Fluor 555 ; AF647 ; Alexa Fluor 647 ; BCA ; bicinchoninic acid ; cdc ; cell division cycle ; CTE ; C-terminal extension ; EM ; electron microscopy ; FFF ; field-flow fractionation ; FFF-MALS ; FFF analyzed by multi-angle light scattering ; FRET ; F&ouml ; rster resonance energy transfer ; MALS ; multi-angle light scattering ; ts ; temperature-sensitive
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：428
• 期：24
• 页码：4962-4980
• 全文大小：1699 K
• 卷排序：428

文摘

Bni5 localizes to the Saccharomyces cerevisiae bud neck in a septin filament-dependent manner. Bni5 oligomerizes and binds cooperatively to septin Cdc11 and requires its C terminus to do so. Cooperative binding of Bni5 also requires the C terminus of Cdc11. Bni5 decorates paired septin filaments with hetero-octameric periodicity. Bni5 binding restrains and constricts the spacing between paired septin filaments.