Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites

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• 作者：Xia Chen ; Xinle Jia ; Jingman Han ; Jie Ma ; Zhanfang Ma
• 关键词：Chitosan-Au nanoparticles ; Carboxyl graphene nanosheet-toluidine blue ; Carboxyl graphene nanosheet-Prussian blue ; Carcinoembryonic antigen ; Alpha-fetoprotein
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 December, 2013
• 年：2013
• 卷：50
• 期：Complete
• 页码：356-361
• 全文大小：1503 K

文摘

In this work, a sandwich-format electrochemical immunosensor for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was fabricated using biofunctional carboxyl graphene nanosheets (CGS) as immunosensing probes, which were fabricated by means of immobilization of toluidine blue (TB) and labeled anti-CEA (Ab_2,1), Prussian blue (PB) and anti-AFP (Ab_2,2) successively on CGS. The capture anti-CEA (Ab_1,1) and anti-AFP (Ab_1,2) were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) modified electrode through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide (EDC/NHS). Experimental results revealed that this sandwich-type immunoassay enabled simultaneous detection of CEA and AFP with linear range of 0.5-60 ng mL^?1 for both analytes. The detection limit was 0.1 ng mL^?1 for CEA and 0.05 ng mL^?1 for AFP (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from the standard ELISA method. And the negligible cross-reactivity between the two analytes allows it to possess potential promise in clinical diagnosis.