Relationships among colony morphotypes, cell-surface properties and bacterial adhesion to substrata in Rhodococcus
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- 作者：Iwabuchi ; Noriyuki ; Sunairi ; Michio ; Anzai ; Hirosi ; Morisaki ; Hisao ; Nakajima ; Mutsuyasu
- 关键词：Extracellular polysaccharide ; Mucoidal or rough mutation ; Hydrophobicity ; Rhodococcus rhodochrous ; Bacterial adhesion
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2003
- 出版时间：July 1, 2003
- 年：2003
- 卷：30
- 期：1-2
- 页码：51-60
- 全文大小：271 K
文摘
To elucidate the function of extracellular polysaccharide (EPS) produced by bacteria with hydrophobic surfaces we investigated relationships among colony morphotypes, cell-surface properties and bacterial adhesion to substrata in Rhodococcus. Rough strains adhered well to various materials and formed cell aggregates, while their mucoidal derivatives did so poorly. Cell surfaces of the rough strains were more hydrophobic than those of the corresponding mucoidal strains. Production of the EPS by the rough strains was far less than that by the corresponding mucoidal strains. Adhesion of the rough strains to quartz, glass or hydrocarbon was inhibited by the addition of EPS. Thus, rhodococcal EPS plays an important role in determination of the cell-surface hydrophobicity by acting as a hydrophilin, and its addition to the rough strains resulted in inhibition of their adhesion to the substrata.