Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate

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• 作者：Wafa Kharroubi¹ ; ² ; ^{wafa.kharroubi@hotmail.com} ; Samia Haj Ahmed¹ ; ² ; Thomas Nury¹ ; Pierre Andreoletti¹ ; Rachid Sakly² ; Mohamed Hammami² ; Gé ; rard Lizard¹
• 关键词：Sodium arsenate ; Microglial BV-2 cells ; Mitochondrial dysfunction ; Oxidative phosphorylation complexes ; Superoxide anions ; Apoptosis
• 刊名：Journal of Environmental Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：51
• 期：Complete
• 页码：44-51
• 全文大小：895 K
• 卷排序：51

文摘

The treatment of microglial BV-2 cells with sodium arsenate (As(V): 0.1–400 μmol/L — 48 hr) induces a dose-dependent response. The neurotoxic effects of high concentrations of As(V) (100, 200 and 400 μmol/L) are characterized by increased levels of mitochondrial complexes I, II, and IV followed by increased superoxide anion generation. Moreover, As(V) triggers an apoptotic mode of cell death, demonstrated by an apoptotic SubG1 peak, associated with an alteration of plasma membrane integrity. There is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate ATP. It is therefore tempting to speculate that As(V) triggers mitochondrial dysfunction, which may lead to defective oxidative phosphorylation subsequently causing mitochondrial oxidative damage, which in turn induces an apoptotic mode of cell death.