Phospholipid-chitosan hybrid nanoliposomes promoting cell entry for drug delivery against cervical cancer
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- 作者：Somsak Saesoo ; Suphawadee Bunthot ; Warayuth Sajomsang ; Pattarapond Gonil ; Sarunya Phunpee ; Patsaya Songkhum ; Kritapas Laohhasurayotin ; Tuksadon Wutikhun ; Teerapong Yata ; Uracha Rungsardthong Ruktanonchai ; Nattika Saengkrit ; nattika@nanotec.or.th" class="auth_mail" title="E-mail the corresponding author
- 关键词：Targeted cancer therapy ; Liposome ; Mucoadhesive nanoparticle ; Cervical cancer
- 刊名：Journal of Colloid and Interface Science
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：480
- 期：Complete
- 页码：240-248
- 全文大小：1227 K
文摘
This study emphasizes the development of a novel surface modified liposome as an anticancer drug nanocarrier. Quaternized N,O-oleoyl chitosan (QCS) was synthesized and incorporated into liposome vesicles, generating QCS-liposomes (Lip-QCS). The Lip-QCS liposomes were spherical in shape (average size diameter 171.5 ± 0.8 nm), with a narrow size distribution (PDI 0.1 ± 0.0) and zeta potential of 11.7 ± 0.7 mV. In vitro mucoadhesive tests indicated that Lip-QCS possesses a mucoadhesive property. Moreover, the presence of QCS was able to induce the cationic charge on the surface of liposome. Cellular internalization of Lip-QCS was monitored over time, with the results revealing that the cell entry level of Lip-QCS was elevated at 24 h. Following this, Lip-QCS were then employed to load cisplatin, a common platinum-containing anti-cancer drug, with a loading efficiency of 27.45 ± 0.78% being obtained. The therapeutic potency of the loaded Lip-QCS was investigated using a 3D spheroid cervical cancer model (SiHa) which highlighted their cytotoxicity and apoptosis effect, and suitability as a controllable system for sustained drug release. This approach has the potential to assist in development of an effective drug delivery system against cervical cancer.