Protection against aflatoxin-B1-induced liver mutagenesis by Scutellaria baicalensis
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- 作者：Johan G. de Boer ; Brendan Quiney ; Patrick B. Walter ; Cynthia Thomas ; Kimberley Hodgson ; Susan J. Murch and Praveen K. Saxena
- 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- 出版年：2005
- 出版时间：2005
- 年：2005
- 卷：578
- 期：1-2
- 页码：15-22
- 全文大小：153 K
文摘
We have measured the inhibition of the mutagenicity of the mycotoxin aflatoxin-B1 in the liver of the rat by plant material of Scutellaria baicalensis, or Huang-qin. The addition of one percent dried Huang-qin to the feed of the animals reduced the mutant frequency of a subsequent administration of aflatoxin-B1 by approximately 60 and 77 % , respectively, for two different batches of the plant material. The addition of Huang-qin also increased the expression of the gene for glutathione S-transferase A5 subunit by 2.5–3.0-fold, and decreased expression of P450 cytochrome 3A2 by 1.8–2.0-fold. The greater increase of the expression of the GST gene may result in the protection shown by Huang-qin. The sensitivity of the hepatic mitochondria to swelling, a measure of the mitochondrial permeability transition, is increased significantly in animals that are on a diet containing Huang-qin. This may lead to increased sensitivity to apoptosis on treatment with toxic compounds. The two batches of Huang-qin material show differences in both chemical composition and preventive potential. This study demonstrates how a combination of generating and analysis of plant varieties together with a mammalian assay for efficacy may improve the search for better plant-based prevention of cancer initiation.