Molecular cloning of kuruma shrimp Marsupenaeus japonicus phosphopyruvate hydratase and its role in infection by white spot syndrome virus and Vibrio alginolyticus
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- 作者：Baozhen Sun1 ; Zhi Wang1 ; Fei Zhu ; zhufei@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Marsupenaeus japonicus ; Phosphopyruvate hydratase ; Gene expression ; Molecular cloning ; White spot syndrome virus
- 刊名：Aquaculture
- 出版年：2016
- 出版时间：20 March 2016
- 年：2016
- 卷：455
- 期：Complete
- 页码：87-96
- 全文大小：2316 K
文摘
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The 1914bp cDNA sequence of phosphopyruvate hydratase was obtained.
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Phosphopyruvate hydratase was highly expressed in muscle of M. japonicus only.
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WSSV or Vibrio alginolyticus infection will change phosphopyruvate hydratase expression tempestuously.
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Phosphopyruvate hydratase is vital for WSSV infection and the dead time of WSSV-infected shrimp was significantly delayed by the gene silencing.