Label-free fluorescent enzymatic assay of citrate synthase by CoA-Au(I) co-ordination polymer and its application in a multi-enzyme logic gate cascade
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- 作者：Yong Li ; Huixia Wang ; Futao Dai ; Pei Li ; Xin Jin ; Yan Huang ; Zhou Nie ; niezhou.hnu@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Shouzhuo Yao
- 关键词：Label-free fluorometry ; Citrate synthase activity assay ; Thiol&ndash ; Au(I) co-ordination polymer ; Logic gate cascade
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：1038-1046
- 全文大小：1939 K
文摘
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Generating RNA-mimicking and SYBR GREEN II stainable coordination polymer of CoA-Au(I) CP in situ.
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Basing on CoA-Au(I) CP, a novel label-free fluorescent-on assay of CS and its inhibitor was developed.
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This CoA-Au(I) CP based method is homogeneous, sensitive, facile, cost-effective, and quick.
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A biomimetic AND-AND-AND-cascaded logic gate was further established in one-pot to process chemical information.