Screening for improved isoprenoid biosynthesis in microorganisms
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- 作者：Anita Emmerstorfer-Augustina ; Sandra Moserb ; Harald Pichlera ; b ; harald.pichler@tugraz.at" class="auth_mail" title="E-mail the corresponding author ; harald.pichler@acib.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：AaADS ; Artemisia annua amorpha-4 ; 11-diene synthase ; AaFS ; Artemisia annua β-farnesene synthase ; CAT ; chloramphenicol acetyltransferase ; DMAPP ; dimethylallyl diphosphate ; DXP ; deoxyxylulose-5-phosphate ; FCM ; fluorescence-activated cell sorting by flow cytometry and magnetic cell sorting ; FPP ; farnesyl diphosphate ; GPP ; geranyl diphosphate ; GGPP ; geranylgeranyl diphosphate ; GGPPS ; GGPP synthase ; IPP ; isopentenyl diphosphate ; HTS ; high-throughput screening ; MEV ; mevalonate ; PPi ; inorganic diphospha
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 October 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：112-120
- 全文大小：1520 K
文摘
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Comprehensive review on screening techniques for enhanced isoprenoid production in microbes.
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Use of whole-cellular systems is clearly favored over cell extracts in isoprenoid screening.
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More direct assessment of isoprenoid production desired in next generation assays.