Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii
详细信息 查看全文
- 作者：Kyle J. Lauersena ; Thomas Baiera ; Julian Wichmanna ; Robin Wö ; rdenwebera ; Jan H. Mussgnuga ; Wolfgang Hü ; bnerb ; Thomas Huserb ; Olaf Krusea ; olaf.kruse@uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：PcPs ; Pogostemon cablin Benth patchoulol synthase ; TAP ; Tris Acetate Phosphate medium ; T2P ; TAP medium without acetate and 2X phosphate content ; FPP ; farnesyl diphosphate ; GPP ; geranyl diphosphate ; GGPP ; geranylgeranyl diphosphate ; CDW ; Cell dry weight ; YFP ; mVenus yellow fluorescent protein ; CFP ; mCerulean3 cyan fluorescent protein ; UQ ; ubiquinone
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：331-343
- 全文大小：3994 K
文摘
- •
Expression of Pogostemon cablin patchoulol synthase in Chlamydomonas reinhardtii.
- •
Expression of E. coli ispA and S. cerevisiae ERG20 in C. reinhardtii.
- •
Localization of native FPP synthase in C. reinhardtii.
- •
223.4 kDa heterologous fusion protein expression in C. reinhardtii.
- •
1.03 mg L−1 phototrophic sesquiterpenoid production from microalgal host.