Nitric oxide and tumor necrosis factor-α production by Ixeris dentata in mouse peritoneal macrophages

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• 作者：Chung ; Hwan-Suck ; Jeong ; Hyun-Ja ; Han ; Mi-Jung ; Park ; Seung-Taeck ; Seong ; Kang-Kyung ; Baek ; Seung-Hwa ; et. al.
• 关键词：Ixeris dentata ; Nitric oxide ; Peritoneal macrophages ; Tumor necrosis factor-α
• 刊名：Journal of Ethnopharmacology
• 出版年：2002
• 出版时间：October, 2002
• 年：2002
• 卷：82
• 期：2-3
• 页码：217-222
• 全文大小：129 K

文摘

Using mouse peritoneal macrophages, we have examined the mechanism by which Ixeris dentata (IXD) regulates nitric oxide (NO) production. When IXD was used in combination with recombinant interferon-γ (rIFN-γ), there was a marked cooperative induction of NO production. However, IXD had no effect on NO production by itself. The increased production of NO from rIFN-γ plus IXD-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-κB). Furthermore, treatment with IXD alone or rIFN-γ plus IXD in peritoneal macrophages caused a significant increase in tumor necrosis factor-α (TNF-α) production. PDTC decreased TNF-α production induced by IXD significantly. These findings demonstrate that IXD increases the production of NO and TNF-α by rIFN-γ-primed macrophages and suggest that NF-κB plays a critical role in mediating these effects of IXD.