The effect of in vivo

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• 作者：Shiro Kawahara ; Narisato Hrai ; Mitsuru Arai ; Norihisa Tatarazako
• 关键词：Co-exposure ; Vitellogenin ; EROD ; Japanese medaka ; Estrogen
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：2009
• 出版时间：January 2009
• 年：2009
• 卷：27
• 期：1
• 页码：139-143
• 全文大小：288 K

文摘

We evaluated the effect of combination chemical exposure on estrogenic activity, in vivo using Japanese medaka (Oryzias latipes) during co-exposure to estrone (E1) and β-naphthoflavone (βNF) or α-naphthoflavone (αNF). We used E1 because which is one of the major estrogenic substances in the environment, and βNF or αNF are both AhR-ligands and usually used as a model substance of common environmental pollutants such as PAHs from physiological response standpoint. The results of present study demonstrate that both βNF and αNF inhibit the E1-induced production of plasma VTG in a concentration-dependent manner. These results indicate that estrogenic activity is suppressed by AhR-ligands in aquatic environment. At the same time, significant increase of EROD was observed in groups of βNF co-exposed with E1, conversely no significant alteration of EROD was observed in groups of αNF co-exposed with E1. Therefore, we conclude that the inhibition of VTG synthesis effect was not directly related to EROD.