Label-free real-time investigation of the effect of telomerase inhibitors based on quartz crystal microbalance measurement
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- 作者：Jie Wang ; Dr.a ; wangjie@ujs.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Lei LiuaAuthor Vitae ; Hongwei Ma ; Prof.b ; hwma2008@sinano.ac.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：QCM ; Telomerase ; Inhibitor ; TRAP
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：239
- 期：Complete
- 页码：943-950
- 全文大小：1983 K
文摘
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We developed a label-free real-time method to detect telomerase activity, which bypasses the potentially disruptive PCR step of traditional methodologies.
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This method provides information not only on the elongation of DNA primers, but also on the length of the elongated-primer molecule.
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We investigated the inhibitory effect of two telomerase inhibitors (EGCG and stavudine) and discuss the mechanism of these inhibitors.