Genetic diversity of Influenza?B virus in 2009-2010 and 2010-2011 in Tunisia

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• 作者：A. El Moussi ; M.A. Ben Hadj Kacem ; J. Ledesma ; F. Pozo ; M. Teresa Cuevas ; I. Casas ; A. Slim
• 关键词：Influenza B ; Mutation ; Phylogeny ; Sequencing ; B/Yamagata lineage ; B/Victoria lineage
• 刊名：Médecine et Maladies Infectieuses
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：43
• 期：8
• 页码：337-344
• 全文大小：3110 K

文摘

Objective

The authors had for aim to characterize influenza?B strains having circulated in Tunisia to identify new mutations and compare them with reference strains.

Methods

The epidemiological surveillance of influenza allowed identifying 19?patients with symptoms related to respiratory infection, who had been infected by influenza B strains isolated in several regions of Tunisia in 2009-2010 and in 2010-2011. Laboratory identification and detection of mutations in the segment encoding hemagglutinin of influenza viruses was performed by real time PCR and sequencing.

Results

The two influenza?B Tunisian strains of the 2009-2010 season belonged to the Victoria lineage, whereas 2010-2011 season strains belonged to B/Victoria/2/87 and B/Yamagata/16/88 lineages with a dominance of the Yamagata lineage (76 % ). This study allowed identifying amino acid substitutions: T121A, S150I, N165Y, T181A, G183R, D196N, S229D, M251V and K253R in the B/Yamagata lineage; L58P, N75K, K109N, N165K, S172P and K257R into the B/Victoria lineage. These mutations were specific of Tunisian groups of variants. Most influenza B-Yamagata lineage viruses (69 % ) were associated with severe cases.

Conclusion

Molecular analysis of the various influenza?B strains circulating in Tunisia is useful to detect new mutations that can modify the phenotype of influenza strains.