Partial contribution of mitochondrial permeability transition to t-butyl hydroperoxide-induced cell death
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- 作者：Xiaolei Shi ; Hikaru Osaki ; Yoshihiro Matsunomoto ; Chisako Fujita ; Daisuke Shinohe ; Naoko Ashida ; Hyunjin Choi ; Yoshihiro Ohta ; ohta@cc.tuat.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：AM ; acetoxymethyl ester ; CsA ; cyclosporin A ; DMEM ; Dulbecco's modified Eagle's medium ; ΔΨm ; mitochondrial membrane potential ; FBS ; fetal bovine serum ; HBS ; HEPES-buffered saline ; MPT ; mitochondrial permeability transition ; t-BuOOH ; t-butyl hydroperoxide ; TMRE ; tetramethylrhodamine ethyl ester ; PPIase ; peptidylprolyl cis-trans isomerase ; ROS ; reactive oxygen species
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：7
- 期：Complete
- 页码：33-38
- 全文大小：1040 K
文摘
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Cell death was induced in C6 cells by 250 μM t-BuOOH.
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Mitochondrial permeability transition (MPT) occurred before cell death.
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MPT was confirmed by observing calcein fluorescence in mitochondria.
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MPT inhibition did not prevent depolarization of mitochondria and cell death.
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Contribution of MPT to cell death is partial under moderate oxidative stress.